Recombination sites in the HLA class II region are haplotype dependent
Gregersen, P.K.; Kao, H.; Nunez-Roldan, A.; Hurley, C.K.; Karr, R.W.; Silver, J.
Journal of Immunology 141(4): 1365-1368
1988
ISSN/ISBN: 0022-1767 PMID: 3135331 Document Number: 317061
We have analyzed DNA sequence polymorphisms of DQ.alpha. and DQ.beta. chains from three haplotypes from the DRw52 family: DR5 DQw1 (FPA, GM3106), DRw6 DQw1 (CB6B, 10w9060), and DRw6 DQw3 (AMALA, 10w9064). The results indicate that the DR5 DQw1 and DRw6 DQw1 haplotypes have arisen by recombination between the DR.beta.1 and DQ.alpha. loci. This contrasts with our previous analysis of DR4 DQ"Wa", DR3 DQ"Wa", and DR7 DQw3 haplotypes, all of which appear to have arisen by virtue of recombination between DQ.alpha. and DQ.beta. Thus, there appear to be at least two different sites where recombination has occurred within the DR and DQ subregions. These differing patterns of recombination were interpreted in the context of the three major family groups of class II haplotypes, the DRw53, DRw52, and DR1/2 haplotype families. The data indicate that haplotypes from these family groups tend to undergo recombination at different locations. We propose that these differences in site of recombination are a reflection of differences in the molecular organization of the haplotypes belonging to each family group.