Ribonucleases and deoxyribonucleases in pancreatic cancer: clinical value and pathophysiological interrelationships
Del Favero, G.; Basso, D.; Fabris, C.; Angonese, C.; Meani, A.; Vianello, D.; Pedrazzoli, S.; Dodi, G.; Naccarato, R.
International Journal of Pancreatology Official Journal of the International Association of Pancreatology 3(Suppl 1): S107-S112
1988
ISSN/ISBN: 0169-4197 PMID: 3209862 Document Number: 313245
Cell wall preparations from the magnesium-dependent halophilic bacterium, Halobacterium volcanii, were studied by high-resolution electron microscopy complemented with image analysis and processing. For ultrastructural studies, specimens were prepared by a variety of methods, including negative staining, and metal shadowing after air-drying, freeze-drying, or freeze-fracturing and etching. All methods revealed the cell wall to be composed of a near-hexagonal lattice of unit cells having a center-to-center spacing of 15.5 nm. While negatively stained samples yielded two types variably revealed the unit cell to be composed of six protomers surrounding a central mass depression. This low-resolution unit cell morphology appears very similar to that of other bacterial cell wall S-layers studied to date.