An analysis of myeloma plasma cell phenotype using antibodies defined at the IIIrd International Workshop on Human Leucocyte Differentiation Antigens
Jackson, N.; Ling, N.R.; Ball, J.; Bromidge, E.; Nathan, P.D.; Franklin, I.M.
Clinical and Experimental Immunology 72(3): 351-356
1988
ISSN/ISBN: 0009-9104 PMID: 3048803 Document Number: 311769
Fresh bone marrow from 43 cases of myeloma and three cases of plasma cell leukemia has been phenotyped both by indirect-rosetting and, on fixed cytospin preparations, by indirect immunofluorescence. Both clustered and unclustered B cell associated antibodies from the IIIrd International Workshop on Human Leucocyte Differentiation Antigens were used. The results confirm the lack of many pan-B antigens on the surface of myeloma plasma cells, i.e. CD19-23, 37, 39, w40. Strong surface reactivity is seen with CD38 antibodies and with one CD24 antibody (HB8). Weak reactions are sometimes obtained with CD9, 10 and 45R. On cytospin preparations CD37, 39 and w40 are sometimes weakly positive, and anti-rough endoplasmic reticulum antibodies are always strongly positive. Specific and surface-reacting antiplasma cell antibodies are still lacking.