Detection of hepatitis A virus RNA by molecular hybridization
Kusov, I.Iu.; Kazachkov, I.A.; Grabko, V.I.; Nastashenko, T.A.; Balaian, M.S.
Voprosy Virusologii 33(5): 548-554
1988
ISSN/ISBN: 0507-4088 PMID: 2851219 Document Number: 311639
Hepatitis A virus (HAV) was propagated in continuous rhesus monkey embryo kidney cells (FRhK-4 line). HAV RNA extracted with phenol from purified HAV pre-treated with proteinase K was used for molecular cloning experiments. Two radioactive plasmids, pHAV-23 and pHAV-5p, containing HAV cDNA fragments complementary to structural and nonstructural parts of HAV genome, respectively, were found to be useful for discrimination between mature virions and subvirus structures. HAV cDNA-RNA hybridization seems to be 10 times more sensitive than the enzyme immunoassay (ELISA).