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Sensitive enzyme immunoassay of cephalexin residues in milk, hen tissues, and eggs

Kitagawa, T.; Gotoh, Y.; Uchihara, K.; Kohri, Y.; Konoue, T.; Fujiwara, K.; Ohtani, W.

Journal - Association of Official Analytical Chemists 71(5): 915-920

1988

ISSN/ISBN: 0004-5756
PMID: 3148610
Document Number: 311253
A sensitive enzyme immunoassay for cephalexin (CEX) was developed using the rabbit antiserum to CEX. .beta.-D-galactosidase-labeled CEX, and a double-antibody separation method. The immunogen of CEX was prepared by coupling the amino group of CEX to thiol groups introduced into bovine serum albumin by the use of N-(m-maleimidobenzoloxy)succinimide as a cross-linker. Highly titered antiserum to CEX was produced in rabbits immunized with the immunogen. Enzyme labeling of CEX with .beta.-D-galactosidase was done by using N-(gamma-maleimidobutyryloxy)succinimide as the cross-linker. The limit of detection was 30 ng CEX/mL sample solution. Application of the method to CEX drug residues detected 30 ng/mL in milk, 60 ng/g in egg yolk, and 400 ng/g in hen tissue.

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