Evaluation of abbreviated enzyme immunoassay method for detection of Salmonella in low-moisture foods
Flowers, R.S.; Klatt, M.J.; Robison, B.J.; Mattingly, J.A.
Journal - Association of Official Analytical Chemists 71(2): 341-343
1988
ISSN/ISBN: 0004-5756 PMID: 3290190 Document Number: 309720
A modified enzyme immunoassay method (EIA) utilizing an 18 h pre-enrichment, a 6-8 h selective enrichment, and a 14 h M-broth post-enrichment is compared to the standard culture method (AOAC/BAM) on selected low-moisture foods. Tested samples included 238 inoculated, 30 naturally contaminated, and 30 uninoculated foods. By EIA, 235 samples were positive (optical densities greater than 0.2 at 405 nm), 233 of which were confirmed culturally. By the culture methods, 221 samples were positive. The EIA method was more productive in detecting salmonellae in inoculated samples of dry cheese powder, chocolate, and nonfat dry milk, whereas the culture method gave better recovery from naturally contaminated meat and bone meal. The modified EIA could be completed in 40 h and required no centrifugation.