Electrogenic character of the functioning of H+-ATPase of synaptic vesicles in the rat brain
Antonikov, I.M.; Glebov, R.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 106(7): 26-29
1988
ISSN/ISBN: 0365-9615 PMID: 3261175 Document Number: 308525
Transmembrane potential (TMP) of synaptic vesicles (SV) from the rat brain was registered by means of potential-sensitive fluorescent probe 3,3' dipropylthiocarbocyanine iodide (dis-C3-(5)). It was found that Mg-ATP generates TMP on the SV membrane suspended in sucrose-buffer medium. TMP did not appear if 0.25 M sucrose medium was replaced by 0.125 M KCl medium or 0.125 NaCl medium, or if the SV membrane was disrupted by triton X-100 or alamethicin, or if there was protonophore m-chlorophenylhydrazone or N-ethylmaleimide influence on SV. The data obtained indicate that functioning of SV proton pump is electrogenic and the level of TMP depends on the presence of anion Cl- in the incubation medium.