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PGF-2 alpha release, progesterone secretion and conceptus growth associated with successful and unsuccessful transcervical embryo transfer and reinsertion in the mare

Sirois, J.; Betteridge, K.J.; Goff, A.K.

Journal of Reproduction and Fertility. Supplement 35: 419-427

1987

ISSN/ISBN: 0449-3087
PMID: 3479596
Document Number: 304566
The outcome of 23 collections and reinsertions of embryos on days 10.5-13.5, of 4 transfers of day-10.5 embryos, and of 13 transfers of day-6.5 embryos (ovulation = day 0) was monitored. Blood samples were taken before and after each procedure to measure plasma 15-keto-13,14-dihydro-PGF2 alpha (PGFM), and then daily for progesterone determination. Mares were also subjected to daily teasing for detection of oestrus, and to uterine ultrasonography for tracing the development of the embryo. After the reinsertions, 12 of 23 embryos were detectable immediately after the procedure. Of these, 1 developed into a normal foal, but 11 disappeared during the next 4 days. All 4 day-10.5 embryos transferred disappeared within 7 days. Of the 13 day-6.5 embryos transferred, 8 were detectable by day 11.5 and 5 of these developed into healthy foals. The 3 losses occurred between days 14 and 25. Small increases in PGFM concentration occurred in 8 of 23 collection/reinsertion procedures on days 10.5-13.5 and after 2 of 25 day-6.5 recoveries, but during none of the 13 day-6.5 transfers. A marked reduction (40%) in plasma progesterone concentration was noted within 24 h of the procedures in only 1 day-10.5 embryo transfer and, in subsequent days, the disappearance of the embryos was associated with complete luteolysis in only 1 day-6.5 transfer, 1 day-10.5 transfer and 1 day-12.5 reinsertion. Embryo growth up to the time of disappearance after reinsertion was normal in 6 mares and retarded in 3; after day-10.5 transfers it was normal; after the 3 day-6.5 transfers that were followed by embryonic loss, growth was normal in 2 and retarded in 1 mare. It appears unlikely that PGF2 alpha release or major reductions in progesterone secretion are implicated in the failure of reinsertions, suggesting that disturbances in the uterine environment or direct adverse effects on the embryo, which do not usually affect its growth, are more likely to blame.

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