Photochemiluminescent detection of antiradical activity. I. Assay of superoxide dismutase
Popov, I.N.; Lewin, G.; von Baehr, R.
Biomedica Biochimica Acta 46(11): 775-779
1987
ISSN/ISBN: 0232-766X PMID: 3446204 Document Number: 303858
The luminol sensitized photogeneration of superoxide radicals was combined with their luminol-dependent chemiluminescent detection. The inhibition of chemiluminescence by superoxide dismutase (SOD) versus enzyme concentration in reciprocals was strongly linear. The amount of SOD notable for 50% inhibition was between 115 and 500 ng for different enzyme preparations.
Document emailed within 1 workday
Related Documents
Kovárová, H.; Stoklasová, A.; Ledvina, M. 1982: The importance of superoxide dismutase in the development of the postirradiation syndrome. X. Superoxide dismutase activity in tissues of irradiated rats protected with cystamine 1 hour after exposure Sbornik Vedeckych Praci Lekarske Fakulty Karlovy Univerzity V Hradci Kralove. Suppl 25(3-4): 357-366Kratochvílová, V.; Krízala, J.; Stoklasová, A.; Ledvina, M. 1981: The significance of superoxide dismutase in the development of th post-irradiation syndrome. VI. the effect of cystamine on superoxide dismutase activity in the erythrocytes of irradiated and non-irradiated rats Sbornik Vedeckych Praci Lekarske Fakulty Karlovy Univerzity V Hradci Kralove. Suppl 24(2): 261-268
Kovárová, H.; Stoklasová, A.; Ledvina, M. 1988: The significance of superoxide dismutase in the development of the post-irradiation syndrome. XII. Activity of superoxide dismutase isoenzymes in the bone marrow of rats after irradiation Sbornik Vedeckych Praci Lekarske Fakulty Karlovy Univerzity V Hradci Kralove. Suppl 31(1): 37-44
Kostyuk, V.A.; Potapovich, A.I. 1989: Superoxide--driven oxidation of quercetin and a simple sensitive assay for determination of superoxide dismutase Biochemistry International 19(5): 1117-1124
Madesh, M.; Balasubramanian, K.A. 1998: Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide Indian Journal of Biochemistry and Biophysics 35(3): 184-188
Zbarskiĭ, I.B.; Peskin, A.V. 1982: Superoxide dismutase activity and the membrane formation of superoxide radicals in tumorous and normal tissues Vestnik Akademii Meditsinskikh Nauk SSSR 9: 24-28
Auclair, C.; Dhermy, D.; Boivin, P. 1981: Intravascular hemolysis in patients with erythrocyte superoxide dismutase deficiency: further evidence for the protective role of superoxide dismutase in aerobic cells Bulletin Europeen de Physiopathologie Respiratoire 17(Suppl): 207-211
Lindenbaum, A.; Magny, J.F.; Abella, A.; Chalas, J.; Baret, A.; Dehan, M. 1987: Toxicity of oxygen and (Cu) superoxide dismutase and (Mn) superoxide dismutase in newborn infants with respiratory distress. Preliminary results Pathologie-Biologie 35(2): 173-176
Agadzhanov, M.I.; Simonian, M.A.; Kazarian, S.A. 1989: The effect of a superoxide dismutase compound on the level of endogenous superoxide dismutase and lipid peroxidation in thermal burns Voprosy Meditsinskoi Khimii 35(4): 28-30
Li, Y.; Kuppusamy, P.; Zweier, J.L.; Trush, M.A. 1996: Role of Cu/Zn-superoxide dismutase in xenobiotic activation. I. Chemical reactions involved in the Cu/Zn-superoxide dismutase-accelerated oxidation of the benzene metabolite 1,4-hydroquinone Molecular Pharmacology 49(3): 404-411
Kakkar, P.; Das, B.; Viswanathan, P.N. 1984: A modified spectrophotometric assay of superoxide dismutase Indian Journal of Biochemistry and Biophysics 21(2): 130-132
García Callejo, F.J.; Blay Galaud, L.; Platero Zamarreño, A.; Velert Vila, M.M.; Mallea Cañizares, I.; Marco Algarra, J. 2000: Detection tonsillar pathology by superoxide dismutase levels. Comparative study of surgical indications for tonsillectomy Acta Otorrinolaringologica Espanola 51(8): 713-718
Naqui, A.; Chance, B. 1986: Enhanced superoxide dismutase activity of pulsed cytochrome oxidase Biochemical and Biophysical Research Communications 136(1): 433-437
Loven, D.P.; Oberley, L.W.; Rousseau, F.M.; Stevens, R.H. 1980: Superoxide dismutase activity in 1,2-dimethylhydrazine-induced rat colon adenocarcinoma Journal of the National Cancer Institute 65(2): 377-381
Zagórski, T.; Dudek, I.; Berkan, L.; Mazurek, M.; Kedziora, J.; Chmielewski, H. 1991: Superoxide dismutase (SOD-1) activity in erythrocytes of patients with multiple sclerosis Neurologia i Neurochirurgia Polska 25(6): 725-730
Yamanaka, N.; Nishida, K.; Ota, K. 1979: Increase of superoxide dismutase activity in various human leukemia cells Physiological Chemistry and Physics 11(3): 253-256
Giełdanowski, J.; Orłowska, B. 1988: Studies on the effect of superoxide dismutase (peroxinorm) on interleukin-1 activity Archivum Immunologiae et Therapiae Experimentalis 36(3): 245-248
Zhang, W.; Hashimoto, K.; Yu, G-Yan.; Sakagami, H. 2002: Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells Anticancer Research 22(1a): 219-224
Lankin, V.Z.; Tikhaze, A.K.; Lemeshko, V.V.; Shermanov, K.; Kaliman, P.A. 1981: Age and changes in the superoxide dismutase and glutathione peroxidase activity of liver cytosol and mitochondria Biulleten' Eksperimental'noi Biologii i Meditsiny 92(9): 310-311
Omaye, S.T.; Potter, B.D.; Poovaiah, B.P. 1986: Alteration of guinea pig erythrocyte superoxide dismutase activity by dietary antioxidants International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 56(2): 161-164