Protein damage after treatment with ozone of protoplasts and isolated E. coli membranes
Matus, V.K.; Kaler, G.V.; Mel'nikova, A.M.; Konev, S.V.
Biofizika 32(3): 477-481
1987
ISSN/ISBN: 0006-3029 PMID: 3304431 Document Number: 298737
The features of ozone-induced damage of E. coli plasma membrane proteins are investigated. A conclusion is made that protein fluorescence quenching is connected with modification of amino acid residues in the vicinity of tryptophane residues. Such modification may be a consequence of reaction with either ozone itself or products of its interaction with membrane lipids and/or proteins. The suggestion of Goldstein and McDonagh that ozone has a predilection for more hydrophilical membrane domains is confirmed. The data obtained are in agreement with a supposition about the leading role of proteins in deleterious action of ozone on cells.