Top Abstract Purchase Related
Home
>
Node
>
Subnode

Isolation and characterization of the alpha and beta chains of human platelet glycoprotein Ib

Canfield, V.A.; Ozols, J.; Nugent, D.; Roth, G.J.

Biochemical and Biophysical Research Communications 147(2): 526-534

1987

ISSN/ISBN: 0006-291X
PMID: 3632685
Document Number: 294968
Human platelet glycoprotein Ib has been purified in milligram amounts from platelets obtained by pheresis of thrombocytotic donors. Purification steps included lectin (wheat germ agglutinin) and immuno (murine monoclonal anti-glycoprotein Ib antibody) - affinity chromatography. The disulfide-linked individual .alpha. and .beta. chains of GPIb were separated and the amino-terminal amino acid sequence of each chain was determined. Rabbit polyclonal antibody directed against each individual chain was prepared by affinity chromatography and shown to be monospecific by western blot analysis using whole platelet lysate as antigen. These studies outline a useful approach to isolate and characterize the individual chains of GPIb.

Document emailed within 1 workday
Secure & encrypted payments

Espevik, T.; Figari, I.S.; Ranges, G.E.; Palladino, M.A. 1988: Transforming growth factor-beta 1 (TGF-beta 1) and recombinant human tumor necrosis factor-alpha reciprocally regulate the generation of lymphokine-activated killer cell activity. Comparison between natural porcine platelet-derived TGF-beta 1 and TGF-beta 2, and recombinant human TGF-beta 1 Journal of Immunology 140(7): 2312-2316
Allione, A.; Wells, V.; Forni, G.; Mallucci, L.; Novelli, F. 1998: Beta-galactoside-binding protein (beta GBP) alters the cell cycle, up-regulates expression of the alpha- and beta-chains of the IFN-gamma receptor, and triggers IFN-gamma-mediated apoptosis of activated human T lymphocytes Journal of Immunology 161(5): 2114-2119
Mikhaĭlov, V.S.; Guliamov, D.B. 1981: Isolation and characterization of DNA polymerase alpha, beta and gamma from the cells of the loach (Misgurnus fossilis) Biokhimiia 46(9): 1539-1547
Closset, J.; Maghuin-Rogister, G.; Combarnous, Y.; Hennen, G. 1973: Primary structure of the subunits of luteinizing hormone (LH); a comparison of amino acid sequence of the alpha and beta chains of LH of ovine, bovine, procine and human origin Archives Internationales de Physiologie et de Biochimie 81(1): 174-176
Kitagawa, H.; Yamamoto, N.; Yamamoto, K.; Tanoue, K.; Kosaki, G.; Yamazaki, H. 1989: Involvement of platelet membrane glycoprotein Ib and glycoprotein IIb/IIIa complex in thrombin-dependent and -independent platelet aggregations induced by tumor cells Cancer Research 49(3): 537-541
Verdaguer, J.; Yoon, J.W.; Anderson, B.; Averill, N.; Utsugi, T.; Park, B.J.; Santamaria, P. 1996: Acceleration of spontaneous diabetes in TCR-beta-transgenic nonobese diabetic mice by beta-cell cytotoxic CD8+ T cells expressing identical endogenous TCR-alpha chains Journal of Immunology 157(10): 4726-4735
Bhalla, K.; Nayak, R.; Grant, S. 1984: Isolation and characterization of a deoxycytidine kinase-deficient human promyelocytic leukemic cell line highly resistant to 1-beta-D- arabinofuranosylcytosine Cancer Research 44(11): 5029-5037
Mohapatra, S.; Chen, Y.; Takata, M.; Mohapatra, S.S.; Sehon, A.H. 1993: Analysis of T-cell receptor alpha beta chains of CD8+ suppressor T cells induced by tolerogenic conjugates of antigen and monomethoxypolyethylene glycol. Involvement of TCR alpha-CDR3 domain in immunosuppression Journal of Immunology 151(2): 688-698
Udagawa, Y.; Armstrong, S.S.; Waites, G.T.; Bell, S.C.; Horne, C.H.; Thomson, A.W. 1985: Immunohistochemical localization of murine alpha 1-pregnancy-associated protein (alpha 1-PAP) in non-pregnant females: a comparative study with human pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) Clinical and Experimental Immunology 61(2): 397-405
Imaoka, S.; Nomura, T.; Ohara, S.; Tamura, Y.; Kosaki, G. 1979: Glycoprotein platelet-specific antigen and beta-thromboglobulin Rinsho Byori. Japanese Journal of Clinical Pathology Suppl 40: 131-137
Hohenegger, M.; Mitterauer, T.; Voss, T.; Nanoff, C.; Freissmuth, M. 1996: Thiophosphorylation of the G protein beta subunit in human platelet membranes: evidence against a direct phosphate transfer reaction to G alpha subunits Molecular Pharmacology 49(1): 73-80
Zhang, W.Y. 1989: Clinical significance of pregnancy-specific beta glycoprotein, human placental lactogen and beta-human chorionic gonadotropin in the serum of patients with trophoblastic diseases Zhonghua Yi Xue Za Zhi 69(4): 206-208; 16
Simonis, S.; Cullen, S.E. 1986: Fatty acylation of murine Ia alpha, beta, and invariant chains Journal of Immunology 136(8): 2962-2967
Ranieri, E.; Gesualdo, L.; Grandaliano, G.; Maiorano, E.; Schena, F.P. 2001: The role of alpha-smooth muscle actin and platelet-derived growth factor-beta receptor in the progression of renal damage in human IgA nephropathy Journal of Nephrology 14(4): 253-262
Arvieux, J.; Roussel, B.; Pouzol, P.; Colomb, M.G. 1993: Platelet activating properties of murine monoclonal antibodies to beta 2-glycoprotein i Thrombosis and Haemostasis 70(2): 336-341
Mizuochi, T. 1992: Structure and function of oligosaccharide chains of the envelope glycoprotein gp120 of human immunodeficiency virus type 1 Nihon Rinsho. Japanese Journal of Clinical Medicine 50(6): 1419-1429
Koning, F.; Lew, A.M.; Maloy, W.L.; Valas, R.; Coligan, J.E. 1988: The biosynthesis and assembly of T cell receptor alpha- and beta-chains with the CD3 complex Journal of Immunology 140(9): 3126-3134
Suzuki, H.; Yamazaki, H.; Tanoue, K. 1992: Platelet structure and function: immunocytochemical localizations of membrane glycoprotein and alpha-granule protein Nihon Rinsho. Japanese Journal of Clinical Medicine 50(2): 218-222
Seftor, R.E.; Seftor, E.A.; Stetler-Stevenson, W.G.; Hendrix, M.J. 1993: The 72 kDa type IV collagenase is modulated via differential expression of alpha v beta 3 and alpha 5 beta 1 integrins during human melanoma cell invasion Cancer Research 53(14): 3411-3415
Chang, A.C.; Salomon, D.R.; Wadsworth, S.; Hong, M.J.; Mojcik, C.F.; Otto, S.; Shevach, E.M.; Coligan, J.E. 1995: Alpha 3 beta 1 and alpha 6 beta 1 integrins mediate laminin/merosin binding and function as costimulatory molecules for human thymocyte proliferation Journal of Immunology 154(2): 500-510