Comparison of different test systems for the radioimmunological determination of the hepatitis B virus surface antigen using monoclonal antibodies
Shchegolev, A.S.; Kushch, A.A.; Momot, A.M.; Shumaĭ, E.P.; Skoblov, I.S.
Voprosy Virusologii 32(5): 544-547
1987
ISSN/ISBN: 0507-4088 PMID: 3433712 Document Number: 294290
The sensitivity of test systems for radioimmunological detection of HBsAg prepared on the basis of polyclonal immunoglobulins, affinity polyclonal antibody (PCA) and monoclonal antibody (MCA) was compared. MCA ZhAK 22 and ZhAK 12 interacting with two different HBsAg determinants: general-type "a" and subtype "y", respectively, were used. The solid phase was sensitized with MCA of each clone or a mixture of MCA in concentration ratios of 1:1, 1:2, and 2:1. After sensitization of the solid phase and addition of HBsAg, 125I-labeled affinity PCA were added (in all tests). The radioimmunological test-system based on MCA ZhAK 22 and ZhAK 12 adsorbed on the solid phase at a 2:1 ratio was found the most sensitive: 0.5 ng, i.e. 4 times as high as that of the system prepared on the basis of PCA.