Membrane cholesterol and phospholipid in human lenses
Fujiwara, H.; Yamamoto, K.; Furutani, A.; Nakata, K.
Nippon Ganka Gakkai Zasshi 91(2): 312-317
1987
ISSN/ISBN: 0029-0203 PMID: 3604820 Document Number: 294014
Membrane preparation was obtained from human cataractous lenses, Christmas tree cataractous lenses and young normal lenses by the method of sucrose density gradient. Membrane cholesterol was measured by high performance liquid chromatography and total phospholipid was measured by Phospholipid-Test Wako. 1. The high cholesterol content and the high cholesterol/phospholipid (c/p) mol ratio in membrane and in insoluble fraction of cataractous lens was revealed. 2. The high cholesterol content of membrane and the high c/p ratio were seen to be similar in cataractous and christmas tree cataractous lenses. 3. These observations suggest that lens membrane fiber displace toward the center of lens by aging and increase high rigidity in cataractous lens.