Impact of changes in intracellular Ca2+ and K+ concentration on the development of hormonal imprinting in a Tetrahymena model system
Köhidai, L.; Muto, Y.; Nozawa, Y.; Csaba, G.
Cellular and Molecular Biology 33(2): 265-274
1987
ISSN/ISBN: 0145-5680 PMID: 3111707 Document Number: 293946
The influence of Ca2+ antagonists (nifedipine, verapamil) and Ca2+ and K+ ionophores (A 23187, valinomycin) on hormonal (insulin) imprinting of Tetrahymena pyriformis Zeuthen and Tetrahymena pyriformis W cells was examined by determination of labeled hormone binding. Reduced intracellular Ca2+ and elevated intracellular K+ equally inhibited hormonal imprinting, probably as a result of various effects in themselves, or in combination, more precisely as the issue of the influences of the agents on membrane structure and polarity, membrane reassembly associated with ion-dependent intracellular events, and certain nuclear level events. Nifedipine, A 23187 and valinomycin diminished the hormone binding capacity of the membrane also in themselves, by a durable effect lasting over several subsequent generations.