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Dependence of membrane protein turnover in Mycoplasma cells on the age of the culture

Aksenov, M.I.; Levina, O.A.; Ladygina, V.G.; Kapitanov, A.B.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 20-24

1987

ISSN/ISBN: 0372-9311
PMID: 3113136
Document Number: 293607
To understand the molecular mechanisms of damages appearing in biological membranes in the process of cellular aging, changes in the rate of catabolic processes in Mycoplasma cells have been studied. This study has revealed that the aging of Acholeplasma laidlawii culture is accompanied by a decrease in the activity of such catabolic enzymes as DNA-ase, RNA-ase, cathepsin D and beta-glucosidase. A considerable increase in the duration of the half-life of membrane proteins has been registered, which is indicative of a decrease in their turnover rate. The electrophoretic separation of membrane proteins has revealed essential changes in their properties. Such decline in the functional activity of the plasma membrane of Mycoplasma cells at the stationary phase is probably due to the inactivation of membrane enzymes and to the decreased rate of their turnover.

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