An evaluation of an enzymatic choline determination for the identification of semen in casework samples
Noppinger, K.; Morrison, R.; Jones, N.H.; Hopkins, H.
Journal of Forensic Sciences 32(4): 1069-1074
1987
ISSN/ISBN: 0022-1198 PMID: 3612062 Document Number: 293193
This study compares the detection of choline in seminal stains by both an enzymatic method and by the standard Florence crystal test. The tests were conducted on 293 actual casework samples. In those samples identified as containing semen, choline was detected twice as often by the enzymatic method compared to the Florence method (84.6 versus 40.3%). The choline results were correlated with spermatozoa and acid phosphatase tests. The enzymatic detection of choline in seminal stains was found to be a fast, easy, sensitive, and reliable test.