Manifestations of domain organization of the Staphylococcus aureus protein a during heat denaturation of its structure
Zinov'eva, L.V.; Sukhomudrenko, A.G.; Denisiuk, A.I.; Zav'ialov, V.P.
Ukrainskii Biokhimicheskii Zhurnal 59(2): 18-21
1987
ISSN/ISBN: 0201-8470 PMID: 3576719 Document Number: 292978
Melting of protein A from Staphylococcus aureus has been studied in neutral medium by the methods of microcalorimetry and circular dichroism. The melting process of protein A is shown to consist of, at least, 5 independent transitions. The transition with the heat absorption maximum at 38 degrees is ascribed to the melting of the C-terminal domain of protein A. The analysis of amino acid sequences has shown the existence of structural basis for differences in heat stability of the Fc-binding domains of protein A.