Plasmid vectors of "insertion inactivation" of the trimethoprim resistance marker
Gorovits, R.L.; Solonin, A.S.
Genetika 23(3): 397-404
1987
ISSN/ISBN: 0016-6758 PMID: 3032740 Document Number: 291229
We demonstrate the possibility of using the T4 phage frd gene as an insertion inactivation marker within pBR322, in plasmids with changing copy number and expression of foreign genes under control. The structural part of the frd genes contains unique recognition sites for EcoRI and SaII endonucleases. Transformants with recombinant plasmids carrying the frd gene grow on media with up to 500 mkg/ml trimetoprim, whatever the gene dosage.