Determination of N-nitroso compounds in the gastric juice for evaluation of the individual nitrosating capacity of the body
Dikun, P.P.; Pavlov, K.A.; Ermilov, V.B.; Volkov, D.P.; Stefanenko, I.F.
Voprosy Onkologii 33(12): 61-67
1987
ISSN/ISBN: 0507-3758 PMID: 3424708 Document Number: 290255
The article deals with a comparison of the results of human body nitrosating ability determination by two methods: (a) nitroso compound (NC) assay in diurnal urine after oral administration of its precursors, and (b) assay of the same in gastric juice after in vitro addition of precursors. The data for the first part of the investigation were obtained from the literature, and for the second one--from the experiments by the authors. Both studies used essentially identical groups of patients, primarily those suffering gastrointestinal pathology. Since the results matched to a considerable degree, it was inferred that (1) gastric juice is a factor of body nitrosating ability, and (2) in vitro determinations of said ability in gastric juice are fully justified. Moreover, the latter procedure is sometimes more practicable, particularly, in view of the marked relationship between nitrosating ability, and, especially, the efficacy of inhibitors of NC endogenous synthesis, on the one hand, and the individual characteristics of the body, on the other.