Expression of selected genes in human hematopoietic cells
Tanaka, Y.; Shiosaka, T.; Sakai, I.; Kobayashi, Y.
Nihon Ketsueki Gakkai Zasshi Journal of Japan Haematological Society 50(4): 854-861
1987
ISSN/ISBN: 0001-5806 PMID: 2446457 Document Number: 290253
Total cellular RNAs from a variety of hematopoietic neoplastic cells and normal lymphocytes were analyzed for homology with 21 recombinant DNA segments complementary to the specific mRNA of cells from a patient with chronic lymphocytic leukemia. Twenty of the clones (except for clone 8-6G) were not significantly represented in the mRNA from normal lymphocytes. The relative concentrations of three clones (6-1E, 7-3G and 9-5C) in the RNAs from a variety of normal and leukemic leukocytes and human hematopoietic cell lines were measured by Northern blot hybridization. This showed that 7-3G, 6-1E and 9.5C RNA were highly abundant in RNA from hematopoietic neoplastic leukocytes.