Morphology of the pig cornea in normal conditions and after incubation in a perfusion apparatus
Camber, O.; Rehbinder, C.; Nikkila, T.; Edman, P.
Acta Veterinaria Scandinavica 28(2): 127-134
1987
ISSN/ISBN: 0044-605X PMID: 3447467 Document Number: 288611
The fine structure of the pig cornea in normal conditions and after being used in a perfusion apparatus, for 4 h, is described. Earlier reports on the normal morphology of the pig cornea were partly not confirmed. Thus the number of cell layers in the epithelium was found to be 19-23 (a basal cell layer, 4-5 polyhedral cell layers and 14-17 squamous cell layers) compared to earlier reported 6-9 layers. The mean thickness of normal and perfused corneas were 722 .mu.m and 752 .mu.m respectively. Normal corneas had a hydration level of 77.2% and after perfusion 78.5%. The normal morphology and morphological changes due to exposure to perfusion were studied by light and electron microscopy. The differences observed betwen normal and perfused corneas have to be considered limited, and restricted mainly to the anterior squamous epithelium and the endothelium. Taken together our results indicate that the corneas used in the apparatus still had functional integrity.