Autophosphorylation of lactate dehydrogenase
Yasykova, M.V.; Ashmarina, L.I.; Muronetz, V.I.; Nagradova, N.K.
Biochemistry International 14(5): 933-938
1987
ISSN/ISBN: 0158-5231 PMID: 3454647 Document Number: 286849
Incubation of rabbit muscle lactate dehydrogenase in the presence of Mg[.gamma.-32p]ATP results in the incorporation of the label into the protein. The autophosphorylation reaction is strongly pH-dependent. The maximal phosphorylation is observed at pH 6.8 with 3-4 moles of phosphate bound per mole of tetrameric enzyme. The enzyme-phosphate complex is readily hydrolyzed by hydroxylamine.
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