Weakening of type i restriction in E. coli: the effect of 2-aminopurine and 5-bromouracil
Belogurov, A.A.; Efimova, E.P.; Del'ver, E.P.; Zavil'gel'skiĭ, G.B.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 12: 34-40
1987
ISSN/ISBN: 0208-0613 PMID: 2833697 Document Number: 286813
2-aminopurine (2-AP) and 5-bromouracil, strong mutagens of base analog type, were found to induce efficiently the alleviation of I type restriction in Escherichia coli. 2-AP induced restriction alleviation occurs in recA, lexA and mut- mutants, but no additional relief of restriction is registered in dam-bacteria in the presence of sublethal 2-AP concentrations. 2-AP specifically alleviates I type restriction in Escherichia coli (EcoA, EcoB, EcoD, and EcoK) and does not affect restriction systems of II (EcoRI) and III (EcoP1) types. We suggest that 2-AP-induced mismatches and other replication errors may be signals inducing restriction alleviation in Escherichia coli.