Chromatographic behavior of the hepatitis B virus surface antigen in donor plasma
Taran, O.I.; Katushkina, N.V.; Zaĭdes, V.M.; Selimova, L.M.; Berezin, V.E.
Voprosy Virusologii 31(4): 457-466
1986
ISSN/ISBN: 0507-4088 PMID: 3765570 Document Number: 283462
Behavior of HBsAg in adsorption chromatography of the antigen-containing plasma in a column with unmodified macropore silica was studied. Under the employed conditions of overlaying and elution, five maximum yields of plasma proper proteins from the column (peaks 1-5) were observed. HBsAg was determined mainly in the material of peaks 4 and 5 in which the dominating host protein was lipoprotein HDL. No HBsAg was detected in albumin-enriched fraction. Similarities of chromatographic behavior of all the three classes of particles whose surface is presented by HBsAg, 20 nm particles, filamentous forms, and Dane particles, were observed.