Bilayer agar cell cultures for studying cellular growth factors
Cherepantseva, E.A.; Shevliagin, V.I.; Al'tshteĭn, A.D.
Biulleten' Eksperimental'noi Biologii i Meditsiny 102(7): 116-119
1986
ISSN/ISBN: 0365-9615 PMID: 3460644 Document Number: 282950
The method for biological testing of growth factors (GF) produced by transformed cells is described. The method is suitable for studying the ability of donor cells to release GF that stimulate colony formation of test-cells. Donor cells and test-cells are placed into different semisolid agar layers and separated by intermediate agar layer. The method provides a much more efficient testing of GF biological activity than the use of conditioned cultural fluids of transformed cells. It permits the assessment of GF dialyzation and the role of donor cell proliferation in the production of GF.