Establishment and characterization of two histologically differentiated human uterine endometrial carcinoma cell lines from the same patient--a correlation between grade of morphological differentiation and HLA antigens
Tanaka, K.; Higuchi, M.; Hando, T.; Yoshiya, N.; Adachi, S.; Tanaka, K.; Honma, S.; Motani, N.; Kanazawa, K.; Honda, T.
Nihon Sanka Fujinka Gakkai Zasshi 38(11): 1975-1981
1986
ISSN/ISBN: 0300-9165 PMID: 3794448 Document Number: 281697
The cell lines designated KKNS-I and KKNS-II were derived from the metastatic lesions in the lymph nodes of endometrial adenocarcinoma. The KKNS-I cells were small spindle or polygonal in shape and showed a jig-saw puzzle-like arrangement with a tendency to pile up. The KKNS-II cells were polygonal in shape, and larger than the KKNS-I. They appeared to be arranged like a pavement. The modal number of the two cell lines was 46 without marker chromosome. Calculating from the growth curves, the doubling times for the KKNS-I and the KKNS-II cells were 35 hours and 60 hours, respectively. The tumors obtained from the nude mouse inoculated with the KKNS-I and the KKNS-II were a poorly differentiated endometrial adenocarcinoma and a well differentiated endometrial adenocarcinoma in histology, respectively. The volume of estrogen receptor and progesterone receptor in the KKNS-II were more than that in the KKNS-I cells. HLA-ABC antigens were detected in both the cells, but HLA-DR antigens were detected in only some populations of KKNS-II cells. These data suggest a relationship between the HLA-DR antigens and the degree of differentiation of the endometrial adenocarcinoma.