1,25-Dihydroxyvitamin D3 inhibits the clonogenic growth of transformed cells via its receptor
Haussler, C.A.; Marion, S.L.; Pike, J.W.; Haussler, M.R.
Biochemical and Biophysical Research Communications 139(1): 136-143
1986
ISSN/ISBN: 0006-291X PMID: 3021122 Document Number: 280604
Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number ov cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 .times. 10-10 M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.