Determination of soluble antigens of Clostridium botulinum a by chemiluminescent--immunosorbent assay (CLISA)
Ligieza, J.; Michalik, M.; Reiss, J.; Grzybowski, J.
Archivum Immunologiae et Therapiae Experimentalis 34(2): 189-195
1986
ISSN/ISBN: 0004-069X PMID: 2431667 Document Number: 280569
The chemiluminescent--immunosorbent assay (CLISA) was adopted for Cl. botulinum A soluble toxic antigens determination. Luminol (ABEI) labelled botulinum antitoxin globulins showed a strongly positive specific immunochemiluminescent reactions with the native botulinum toxin preparations coupled (adsorbed) on polystyrene balls. The sensitivity of the reaction reached 20 DLM/ml (5,000 light impulses per 40 sec) in comparison with 2 X 10(6) DLM (57,000 impulses), control preparations (1,500 impulses) and the background (150-300 impulses). The results give a perspective further investigations with the CLISA for rapid indication of Cl. botulinum toxic antigens.