Effect of calcium channel blockers on myocardial infarct size in rat
Singh, N.K.; Singh, M.
Indian Journal of Experimental Biology 24(4): 252-255
1986
ISSN/ISBN: 0019-5189 PMID: 3770815 Document Number: 277407
Infarction was produced in rats by left coronary artery ligation (CAL) and infarct size was measured using NBT (nitroblue tetrazolium chloride) staining technique. Clearly demarcated (dark stained, lightly stained and non-stained) zones were observed after 72 hr of CAL, and maximum infarct size (non-stained zone) was noted at this time period. Thus 72 hr after CAL was observed to be most appropriate time to evaluate the effect of drugs on infarct size in rat. Verapamil and nifedipine administered ip decreased the infarct size, but no such protective effect was noted when they were administered orally. Tiapamil administered ip was also found to be effective to limit the infarct size. During treatment increased the size of the slightly stained zone and simultaneously decreased the size of non-stained zone. Calcium channel blockers perhaps through their metabolic effects affected the non-functional tissue to regain its cellular integrity.