Glyburide action in cultured 3T3-L1 adipocytes
Miller, R.E.; Bhandari, B.
Biochemistry International 13(2): 313-319
1986
ISSN/ISBN: 0158-5231 PMID: 2876707 Document Number: 277222
During adipocyte differentiation of 3T3-L1 cells, glyburide increased the specific activity (mU/mg protein) of glycerol-3-P dehydrogenase (by at least 14-fold) and glutamine synthetase (by 5-fold). The glyburide-mediated increases in enzyme activities were greater in the presence than in the absence of insulin. Our data indicate that glyburide either potentiates or mimics the actions of insulin to increase the activity of glycerol-3-P dehydrogenase during adipocyte differentiation of cultured 3T3-L1 cells.