Comparison of extraction methods for methamphetamine and its metabolites in tissue
Inoue, T.; Suzuki, S.
Journal of Forensic Sciences 31(3): 1102-1107
1986
ISSN/ISBN: 0022-1198 PMID: 3734729 Document Number: 276738
Five extraction methods were examined for analysis of methamphetamine and its major metabolites in tissue samples. The extraction methods studied were an acetone extraction method, an ethanol extraction method, an ammonium sulfate method, dialysis, and a direct solvent extraction. Acetone, ethanol, and dialysis methods showed no interference from endogenous components using thin-layer chromatography and gas chromatography, and gave satisfactory recovery of methamphetamine, amphetamine, and p-hydroxymethamphetamine when added to rabbit liver. These methods, however, proved time-consuming. The ammonium sulfate method and direct solvent extraction method were simple and more rapid, but recovery of the polar metabolite was poor.