Study of the chondriome in SPEV cell culture after treatment with actinomycin D
Shornikova, M.V.; Bykov, A.M.; Chentsov, I.S.
Tsitologiia i Genetika 20(6): 403-409
1986
ISSN/ISBN: 0564-3783 PMID: 3810850 Document Number: 275864
It is shown that the 12-hour treatment of cell with actinomycin D (AMD) in the concentration of 0.05 microgram/ml disturbs a correlation between the morphological cycle of mitochondria and phases of the mitotic cell cycle which is characteristic of intact cells. An increase in the total number of mitochondria independent of phase is observed in all the cells in comparison with intact cells. At the same time a decrease in the amount of branched organellae and appearance of giant mitochondria are discovered. All mitochondria are in the condense form. These changes, perhaps, are a result of the inhibition of the rRNA synthesis in the nucleus and of the protein synthesis in the cell found with it by AMD. The possibility of the immediate interaction of AMD with membrane components of the cell, which induces changes in the ion concentrations and peroxidation of the membrane lipids is not excluded.