Syntheses and effects of [Glu34]-thymopoietin Ii fragment 32-45 and its analogs on the impaired T-cell transformation in a patient with common variable immunodeficiency
Abiko, T.; Shishido, H.; Sekino, H.
Biotechnology and Applied Biochemistry 8(2-3): 160-171
1986
ISSN/ISBN: 0885-4513 PMID: 3488750 Document Number: 273695
[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of m-cresol. These peptides were tested for their effects on impaired T-cell transformation by phytohemagglutinin in the common variable immunodeficiency. The relative potency of the synthetic [Glu34]-thymopoietin II fragment 32-45 was one-half of that of synthetic thymopoietin II fragment 32-45. Among these tetradecapeptide analogs, one analog in which Val37 was replaced by Ile exhibited a potent activity which was more than that of the synthetic [Glu34]-thymopoietin II fragment 32-45. The relative potencies of Thr37 and Tyr37 analogs were one-third and one-half, respectively of that of the synthetic [Glu34]-thymopoietin II fragment 32-45.