Effects of recombinant human interferon gamma on hematopoietic progenitor cell growth
Toretsky, J.A.; Shahidi, N.T.; Finlay, J.L.
Experimental Hematology 14(3): 182-186
1986
ISSN/ISBN: 0301-472X PMID: 3081359 Document Number: 273169
Human bone marrow BFU-E, CFU-E, and CFU-GM were cultured in the presence of varying concentrations of recombinant human interferon gamma (rHuIFN-.gamma.). Concentration-dependent inhibition of both erythroid and myeloid precursors by rHuIFN-.gamma. was demonstrated. A more pronounced suppressive effect of rHuIFN-.gamma. was seen on the BFU-E than on the CFU-E, with CFU-GM most resistant. rHuIFN-.gamma. was also added at varying time points during the marrow cultures, demonstrating different time-dependent sensitivities to rHuIFN-.gamma.; CFU-E were no longer sensitive to rHuIFN-.gamma. by day 2 of culture, BFU-E by day 6, and CFU-GM by day 9, indicating a loss of sensitivity with maturation. Finally, exposure of marrow cells to rHuIFN-.gamma. for varying periods of time prior to initiation of hematopoietic cultured failed to inhibit erythroid colony growth in the absence of rHuIFN-.gamma. in the culture. These studies demonstrate a suppressive effect of rHuIFN-.gamma. on human erythroid and myeloid progenitor cell growth. This effect appears to be most pronounced on the more primitive stages of committed progenitor cell development.