Isolation and fractionation of the nuclear matrix from cultured cells
Chernokhvostov, V.V.; Cherni, N.E.; Moskvin-Tarkhanov, M.I.; Razin, S.V.
Tsitologiia 28(8): 843-847
1986
ISSN/ISBN: 0041-3771 PMID: 3535185 Document Number: 273031
A new method for the isolation of tissue culture cell nuclei is presented which involves incubation of the nuclei in the presence of Cu2+- or Zn2+-ions. This method eliminates the danger of nuclear aggregation and permits nuclear matrix isolation and subsequent fractionation. Stabilization of the inner matrix by Cu2-ions permits analysis of the role of nucleic acids in the maintenance of the matrix structure. It is shown that solubilization of more than 95% of matrix-bound DNA and more than 90% of matrix-bound RNA did not cause any significant changes in the nuclear matrix structure.