Effect of melanin bleaching on immunoperoxidase, with reference to ocular tissues and lesions
Alexander, R.A.; Hiscott, P.S.; Hart, R.L.; Grierson, I.
Medical Laboratory Sciences 43(2): 121-127
1986
ISSN/ISBN: 0308-3616 PMID: 2426554 Document Number: 272706
Naturally or pathologically occurring brown pigmented accumulations of melanin or haemosiderins may cause problems of interpretation when diamino benzidine is used to visualise the final reaction product of immunoperoxidase procedures. A 2.5 g/l solution of potassium permanganate bleaches melanin without affecting the subsequent immuno-staining of a number of cell markers used in ophthalamic histopathology-keratin (epithelial cells), glial fibrillary acidic protein (glial cells) and factor VIII-related antigen (vascular endothelial cells). Permanganate has also been found abolish endogenous peroxidase activity.