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Clonogenic stromal cell count in the bone marrow of mice

Latsinik, N.V.; Gorskaia, I.F.; Pavlenko, R.G.; Fridenshteĭn, A.I.

Biulleten' Eksperimental'noi Biologii i Meditsiny 101(4): 468-471

1986

ISSN/ISBN: 0365-9615
PMID: 3697495
Document Number: 272550
The number of fibroblast colonies in bone marrow cultures depends on FCFC concentration in explanted cells and FCFC cloning efficiency. For mouse bone marrow the efficiency of fibroblast colony formation increases in the presence of the feeder (irradiated bone marrow of spleen cells). Colony-stimulating feeder activity does not depend on the presence of phagocytic and stromal cells in the feeder cell population. Trypsinization of the bone marrow leads to the release of additional FCFC and the increase of their concentration in bone marrow cell suspensions.

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