Reverse electrical pacing improves intestinal absorption and transit time
Sawchuk, A.; Nogami, W.; Goto, S.; Yount, J.; Grosfeld, J.A.; Lohmuller, J.; Grosfeld, M.D.; Grosfeld, J.L.
Surgery 100(2): 454-460
1986
ISSN/ISBN: 0039-6060 PMID: 3488600 Document Number: 271709
In 25 male Sprague-Dawley rats (148 to 208 g) which underwent division and anastomosis of the proximal jejunum to eliminate the proximal gastroduodenal pacemaker, a 30.0-cm loop ileostomy was formed and leads were placed at 1.0 and 3.0 cm proximal to the stoma. Reverse pacing was done with a 0.25 Hz, 50 msec pulse at 0.1 mA. Transit time was evaluated and was 12 +or- 4 min in group I (13 controls) and 27 +or- 21 min in group II (12 reverse-paced rats). D-Xylose absorption, estimated in 18 rats, was 14.0 +or- 3.6 mg/100 ml in 6 control rats and 15.5 +or- 3.4 mg/100 ml in 6 reverse-paced rats. Increasing the pulse milliamperage to 2.0 mA (6 rats) increased D-xylose in serum to 38.8 +or- 27.7 mg/100 ml. Transit rate and net water flux were determined in 8 additional rats with 15 cm Theiry-Vella loops. Transit rate was 3.00 +or- 2.32 ml/min in unpaced rats compared with 9.95 +or- 0.71 ml/min with reverse pacing. Water flux studies showed that control rats had a net secretory loss of 0.20 +or- 0.48 ml/cm whereas paced rats absorbed 0.08 +or- 0.12 ml/cm.