Single-radial-immunodiffusion potency tests of inactivated influenza vaccines for use in man and animals
Wood, J.M.; Mumford, J.; Schild, G.C.; Webster, R.G.; Nicholson, K.G.
Developments in Biological Standardization 64: 169-177
1986
ISSN/ISBN: 0301-5149 PMID: 3098605 Document Number: 271584
Single-radial-immunodiffusion (SRD) provides a sensitive and reproducible in vitro assay for haemagglutinin (HA) concentration in inactivated influenza vaccines. The use of SRD for human influenza vaccine standardization and application for equine and avian influenza vaccines is discussed. In clinical trials, vaccine HA concentration measured by SRD has been shown to be directly related to antibody responses and to protection against challenge. The use of SRD may considerably reduce the usage of animals for potency testing of veterinary influenza vaccines.