Activity of native and dextran-modified hyaluronidase in in vitro and in vivo systems
Maksimenko, A.V.; Konovalova, O.I.; Berdichevskiĭ, V.R.; Arkhipova, O.G.; Torchilin, V.P.
Biulleten' Eksperimental'noi Biologii i Meditsiny 102(11): 567-569
1986
ISSN/ISBN: 0365-9615 PMID: 2430642 Document Number: 270575
Modified hyaluronidase derivatives have been obtained. Covalent coupling of the enzyme with aldehyde dextran results in 65-85% protein binding to the carrier, residual catalytic activity accounting for 90-100% of the baseline. Modified hyaluronidase is more thermostable than the native enzyme. The data on intravenous drug distribution in the mouse organs are promising and ensure effective use of modified hyaluronidase for the treatment of pulmonary diseases.