Cytogenetic effects of diesel engine exhausts on mammalian cells in vitro and in vivo
Sofuni, T.; Hayashi, M.; Matsuoka, A.; Sawada, M.; Hatanaka, M.; Ishidate, M.
Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences 1986(104): 97-103
1986
ISSN/ISBN: 0077-4715 PMID: 2436699 Document Number: 270463
The extract of diesel exhaust particles was examined in a chromosomal aberration test with cultured Chinese hamster cells (CHL), and induced chromosomal aberrations in both the presence and absence of S9 mix. The induction of sister chromatid exchanges (SCEs) was also examined in the same test substance, and positive results were obtained in both the presence and absence of S9 mix. The micronucleus tests on rats exposed to diesel engine exhaust for 3, 6 and 24 months were carried out using bone marrow cells and peripheral blood. There was no significant increase in the frequencies of micronucleated erythrocytes in both the bone marrow and peripheral blood. In addition, when chromosomal aberrations in alveolar macrophages of mice exposed to the diesel exhaust for 1 to 21 days were examined, a tendency toward a slight increase in frequencies of chromosomal aberrations in alveolar macrophages was found with the length of the exposure period. The significance of the comparative cytogenetic effects of diesel engine exhaust on in vitro and in vivo experiments in mammalian cells was discussed.