Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism
Makarov, S.A.; Kudriavtseva, G.V.; Shishkin, V.I.
Voprosy Meditsinskoi Khimii 32(6): 126-128
1986
ISSN/ISBN: 0042-8809 PMID: 3811277 Document Number: 270437
A procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. The procedure involved polarographic registration of oxygen consumption, which is directly related to content of NADPH formed, in a coupled enzymatic system containing peroxidase and Mn2+.
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