Comparison between sperm treated with pentoxifylline and 2-deoxyadenosine using hypo-osmotic swelling test
Laokirkkiat, P.; Kunathikom, S.; Choavaratana, R.; Petyim, S.; Prechapanich, J.
Journal of the Medical Association of Thailand 90(2): 211-215
2007
ISSN/ISBN: 0125-2208 PMID: 17375622 Document Number: 2698
Objective: To compare the percentage of sperm tail membrane swelling under hypo-osmotic conditions between sperm treated with pentoxifylline and 2-deoxyadenosineDesign: Experimental in vitro studyMaterial and Method. Thirty normal semen samples from wale partners of infertile couples were collected After sperm preparation by two-layer Percoll gradient method, each sperm sample was divided into three specimens. Pentoxifylline and 2-deoxyadenosine were separately added into two specimens, while the third specimen was used as a control. Hypo-osmotic swelling test was performed in all specimens. Percentage of swollen spermatozoa in each specimen was evaluatedResults: The mean percentage of swollen spermatozoa in the semen samples supplemented with pentoxifylline and 2-deoxyadenosine were both significantly higher than those in the control (82.8 +/- 7.7 and 83.0 +/- 9.5 vs 70.8 +/- 12.7; p < 0.001). There was no significant differences of swollen spermatozoa between pentoxifylline and 2-deoxyadenosine (p = 0. 898).Conclusion: Addition of pentoxifylline and 2-deoxyadenosine to the sperm prepared by the two-layer Percoll gradient method can almost equally enhance the sperm membrane integrity. Therefore, it may be beneficial to add these compounds to sperm preparation for use in assisted reproduction.
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