Transmission of chronic idiopathic vitritis in mice by inoculation of human vitreous containing leucocyte phagolysosomal bacteria-like bodies
Wirostko, E.; Johnson, L.A.; Wirostko, B.M.
Lancet 2(8505): 481-483
1986
ISSN/ISBN: 0140-6736 PMID: 2875238 Document Number: 267832
Vitreous humour from chronic idiopathic vitritis (CIV) patients containing 0.5-0.7 .mu.m diameter bacteria-like bodies (BLB) in polymorphonuclear leucocytes was inoculated into the eyelids of 100 mice. 200 control mice received either eye-bank vitreous or saline. After 12 months, 53 mice that received CIV vitreous, but none of the controls, had clinical signs of ocular inflammation (p < 0.05); 15 of the mice that received CIV vitreous and none of the controls had histological evidence of chronic deep ocular inflammation, including vitritis (p < 0.05); 95 CIV-vitreous-inoculated and 38 control mice were dead (p < 0.05); and 3/3 of the CIV-vitreous group, compared with 0/3 controls, that were killed for histological assessment had phagolysosomal BLB identical to those in the CIV-vitreous inocula. The findings indicate that the BLB are pathogenic for mice.