Binding characteristics of swine erythrocyte insulin receptors
Dieberg, G.; Bryan, G.S.; Sartin, J.L.; Williams, J.C.; Prince, T.J.; Kemppainen, R.J.
American Journal of Veterinary Research 46(9): 1929-1933
1985
ISSN/ISBN: 0002-9645 PMID: 2996389 Document Number: 266005
Crossbred gilts (controls; n = 7) had 8.8 .+-. 1.1% (mean .+-. SEM) maximum binding of [125I]insulin to insulin receptors on erythrocytes. The number of insulin-binding sites per cell was 137 .+-. 19, with binding affinity ranging from 7.4 .times. 107M-1 to 11.2 .times. 107M-1 and mean of 8.8 .times. 107M-1. Pregnant sows (n = 5) had a significant increase (P < 0.01) in maximum binding due to an increase in number of receptor sites per cell. Lactating sows fed a high-fiber diet (n = 3) and a low-fiber diet (n = 4) did not develop a significant difference in maximum binding for insulin. Sows fed the low-fiber diet had a significantly higher number of binding sites and a significantly lower binding affinity than did sows fed a high-fiber diet. Receptor-binding affinity was lower in the low-fiber diet group than in cycling gilts, whereas data from sows fed the high-fiber diet did not differ from data for cycling gilts. Data from this study indicated that insulin receptors of swine erythrocytes have binding characteristics similar to those in other species. Pregnancy and diet will alter insulin to receptor binding in swine.