Cytophotometric analysis of the chromatin structural conformity in interphase nuclei detected in UV light and by gallocyanine staining
Zhukotskiĭ, A.V.; Shchegolev, A.I.; Butusova, N.N.; Nemirovskiĭ, L.E.; Kogan, E.M.
Biulleten' Eksperimental'noi Biologii i Meditsiny 99(6): 726-727
1985
ISSN/ISBN: 0365-9615 PMID: 2410060 Document Number: 264750
Geometric and optical parameters of chromatin of hepatocyte nuclei have been examined before (UV, lambda = 265 nm) and after gallocyanine staining. Quantitative parameters of the chromatin structure in the same nuclei measured in situ by a scanning microscope-photometer (step size 0.125 micron) before and after staining were equal. Tinctorial properties of chromatin granules (condensed part of the nuclear material) and its diffuse part were different. It is suggested that the difference between granules and the nongranular part of chromatin is not only of optical but also of chemical nature.