Equilibrium characteristics of calcium binding on the inner surface of membrane vesicles
Kosterin, S.A.; Bratkova, N.F.
Biofizika 30(3): 436-440
1985
ISSN/ISBN: 0006-3029 PMID: 4027272 Document Number: 264055
A method is proposed for determining equilibrium parameters of calcium binding on the inner surface of membrane vesicules. Based on the studies of concentration relationship of calcium content in vesicules of myometrium sarcolemma (at the equilibrium state) there were determined the values of maximal capacity of Ca2+-binding (12.79 nmole/mg of protein) and the dissociation constants of Ca2+-binding centre complex (62.3 mkM) on the inner surface of the vesicules, and the value of intravesicular volume as well (25.7 mkl/mg of protein).