Role of a carbohydrate component in the formation of immunochemical determinants of carcinoembryonic antigen
Pavlenko, A.F.; Kurika, A.V.; Belogortseva, N.I.; Nabiullin, A.A.; Ovodov, I.S.
Bioorganicheskaia Khimiia 11(5): 646-650
1985
ISSN/ISBN: 0132-3423 PMID: 2412566 Document Number: 260038
Carcinoembryonic antigen (CEA) isolated from metastases of colon adenocarcinoma was subjected to deglycosylation with liquid hydrogen fluoride. The protein fraction obtained (PF CEA) was used for to prepare monospecific antiserum to PF CEA. Comparative studies of CEA, PF CEA, and non-specific cross-reacting antigen (NCA-1) have been carried out using monospecific antisera. Circular dichroism spectra of CEA and PF CEA have been studied. The data obtained suggest that some immunodominant regions of CEA are topographic, and their formation needs a specific conformation of the macromolecule, which is stabilized by the sugar moiety.