Decapreno-beta-carotene as an internal standard for the quantification of the hydrocarbon carotenoids by high-performance liquid chromatography
Khachik, F.; Beecher, G.R.
Journal of Chromatography 346: 237-246
1985
ISSN/ISBN: 0021-9673 PMID: 3841351 Document Number: 259014
The application of decapreno-.beta.-carotene as an internal standard in quantification of the hydrocarbon carotenoids extracted from raw carrots has been thoroughly examined. Decapreno-.beta.-carotene is a C50 .beta.-carotene that has most of the requirements of an internal standard and it can be commercially synthesized in high purity. An isocratic high-performance liquid chromatographic (HPLC) system has been developed that separated all-trans-.alpha.-carotene, all-trans-.beta.-carotene, and its 15,15'-cis-isomer from this internal standard. Quantitative determination of the hydrocarbon carotenoids in carrots by HPLC using the internal standard technique gave values for .alpha.- and .beta.-carotene similar to those obtained from .alpha.- and .beta.-carotene standards alone.