In vitro antigenic reactivity of synthetic lipid A analogues as determined by monoclonal and conventional antibodies
Kasai, N.; Arata, S.; Mashimo, J.; Okuda, K.; Aihara, Y.; Kotani, S.; Takada, H.; Shiba, T.; Kusumoto, S.
Biochemical and Biophysical Research Communications 128(2): 607-612
1985
ISSN/ISBN: 0006-291X PMID: 2581565 Document Number: 258637
Cross-reactivities of synthetic lipid A analogs with monoclonal and conventional antibodies against Salmonella lipid A were studied. The in vitro antigenicity of synthetic compound 506, .beta.-(1 .fwdarw. 6) D-glucosamine disaccharide 1,4'-bisphosphate, which is acylated at 2'-amino and 3'-hydroxyl groups with (R)-3-dodecanoyloxytetradecanoyl and (R)-3-tetradecanoyloxytetradecanoyl groups, respectively, and has (R)-3-hydroxytetradecanoyl groups at 2-amino and 3-hydroxyl groups, was practically indistinguishable from that of the natural Escherichia coli lipid A preparation. Both phosphates in positions 1 and 4' and ester- and amide-linked fatty acyl residues, particularly the 3-acyloxyacyl group, of the glucosamine disaccharide are involved in the cross-reactivity of lipid A as important antigenic determinants.